mouse igg vector lab Search Results


trueview autofluorescence  (Vector Laboratories)
96
Vector Laboratories trueview autofluorescence
Trueview Autofluorescence, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr amplicon  (New England Biolabs)
97
New England Biolabs pcr amplicon
Pcr Amplicon, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent secondary antibodies  (Vector Laboratories)
96
Vector Laboratories fluorescent secondary antibodies
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Fluorescent Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat antirabbit antiserum  (Vector Laboratories)
96
Vector Laboratories biotinylated goat antirabbit antiserum
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Biotinylated Goat Antirabbit Antiserum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse plasma  (Taconic Biosciences)
90
Taconic Biosciences mouse plasma
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Mouse Plasma, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc kit  (Vector Laboratories)
96
Vector Laboratories vectastain abc kit
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ba 2001  (Vector Laboratories)
96
Vector Laboratories ba 2001
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Ba 2001, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mounting media  (Vector Laboratories)
96
Vector Laboratories mounting media
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated horse anti mouse igg antibody  (Vector Laboratories)
96
Vector Laboratories biotinylated horse anti mouse igg antibody
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Biotinylated Horse Anti Mouse Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 6 diamidino 2 phenylindole  (Vector Laboratories)
98
Vector Laboratories 4 6 diamidino 2 phenylindole
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
4 6 Diamidino 2 Phenylindole, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain elite abc kit  (Vector Laboratories)
96
Vector Laboratories vectastain elite abc kit
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Vectastain Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti brdu antibody fluorescent streptavidin mouse anti neun antibody anti mouse igg conjugated  (Vector Laboratories)
99
Vector Laboratories mouse anti brdu antibody fluorescent streptavidin mouse anti neun antibody anti mouse igg conjugated
Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Mouse Anti Brdu Antibody Fluorescent Streptavidin Mouse Anti Neun Antibody Anti Mouse Igg Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.

Journal: Journal of Biological Chemistry

Article Title: Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model

doi: 10.1016/j.jbc.2021.100482

Figure Lengend Snippet: Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.

Article Snippet: Chem. (2021) 296 100482 After permeabilization and blocking, sections were incubated with primary antibodies (anti-insulin (Cat #66198-1-Ig, Proteintech Group, Inc); anti-glucagon (Cat # 15954-1- AP,Proteintech Group, Inc)) overnight at 4 C and incubated with appropriate fluorescent secondary antibodies (DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse, Vector Laboratories) for detection.

Techniques: Generated, Microscopy, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation